Avian Cell Bank Characterization

Before the development of cell culture as a production matrix, vaccines were exclusively derived from viruses grown in animal systems. Further research into the potential of viruses to grow in cultivated cells led to the use of cells from several mammalian and avian sources as production matrixes in vaccine research and development. The avian cell banks are suitable for the production of a variety of viruses and vectors, they can replace chicken embryo fibroblasts (CEF) as a substrate, or the cultivation of viruses in embryonated hen's eggs. In addition, these cell banks can be used for different other products, such as sources of animal proteins or even for food applications.

According to current regulations, Creative Bioarray provides comprehensive and detailed services for avian cell bank characterization to help our customers worldwide in the field of cell banks, which are well characterized and have been tested for sterility, mycoplasma, extraneous agents, tumorigenicity, etc. Rapid and accurate results will be delivered to assist customers in accessing the quality of cell banks as soon as possible.

Our Cell Bank Characterization Services Cover the Following Aspects

• The origin and history of the cell line cellular morphology and growth characteristics, including date of tissue origin, species, sex, age, donor normal or abnormal health status, the generation number of sub-cultured cells, etc.
• Culture medium. Culture medium type and name, serum source, and content.
• Cryopreservation solution. Name of culture and lyophilization solution.
• Cell viability testing. Cell viability and growth characteristics before and after cryopreservation.

• Cell morphology type detection. Epithelial cells, fibroblasts, lymphocytes or other cells, etc.
• Karyotype analysis. Diploid or polyploid, with or without marker chromosomes.
• No contamination detection. No contamination with bacteria, fungi, mycoplasma, protozoa, and viruses.
• Species detection. Detection of isoenzymes to demonstrate that cells are free of cross-contamination.
• Immunoassays, including but not limited to 1-2 serological tests.
• Chromosome band type analysis. Cellular germline origin was determined by the distribution and number of G-bands, C-bands, Q-bands, and Ag-NORs, as well as deterministic chromosomes brought about by replication.
• Expression assay of some genes in cultured cells. It is mainly used to detect whether cultured cells perform their normal functions in gene expression.
• Comparative study of homozygous homologous sequences of cultured cells and fresh animal tissues. Comparative sequence analysis is used to determine whether mutations occur in the genes of cultured cells.
• Detection of the growth rate of cultured cells. Determine the growth pattern of different species of cells and identify the cell growth and development status.
• Culture cell count assay. Determine the number of cells at the time of freezing and ensure successful cell recovery.

As a pioneer in biosafety testing service, Creative Bioarray provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We offer international testing methodologies for avian cell bank characterization. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.