Cell-Based Validation

Cell-Based Validation

Cell-based assays are important experimental tools in life science research and biomanufacturing. They are based on cell culture methods in which living cells are grown in vitro and used as a model system to assess the biochemistry and physiology of healthy and diseased cells. Cell culture assays provide a method to quantitatively analyze the presence, number, or functional activity of cells or tissues of interest. Both standardized and customized cell-based assays are widely used by scientists for numerous applications as cost-effective and reproducible research tools. For example, cell culture assays are critical in preclinical drug discovery, and recently emerging technologies such as 3D cell culture and pluripotent stem cells help improve compound efficacy and toxicity screening predictions.

The Features of Our Cell Validation

Our scientific team works with you to determine the best assay design for your needs. Our assay development experience covers a broad range of research objectives, tailoring each test study for sponsor relevance by selecting the correct cellular system, stimuli, time points, and endpoint readouts to fit the research objectives. We have experience working with a variety of cellular backgrounds, from easy-to-transfect cell lines (e.g., HEK 293, 293FT, HCT116, or HeLa) to difficult-to-transfect cell lines (e.g., fibroblasts, neuroblastoma-derived, hepatocytes carcinoma, bone marrow-derived and endothelial-derived cells).

  • The largest collection of clones that best suits your needs
  • Rigorous and rigorous experimental protocols to ensure quality results
  • Detailed reports and data sheets to aid in decision making
  • Complete services to support your research needs

The Features of Our Cell Validation

Cell-Based Validation Services

Cell Contraction Assays Cell Health Cell Migration, Invasion and Wound Healing
  • Cell contraction assays, floating matrix model
  • Cell contraction assays, two-step attached model
  • Anchorage-dependent cell death
  • Autophagy
  • Cell proliferation assays
  • Cellular senescence assays
  • Cytotoxicity assays
  • Boyden chamber assays
  • Gap closure assays
Phagocytosis Assays Stem Cell Research Cell Transformation
  • Phagocytosis assay, E. coli substrate
  • Phagocytosis assay, red blood cell substrate
  • Phagocytosis assay, zymosan substrate
  • Alkaline phosphatase detection
  • Feeder cells
  • Hematopoietic CFC assays
  • Stem cell colony formation assay
  • 96-well cell transformation assays, soft agar with cell recovery
  • 96-well cell transformation assays, standard soft agar
shRNA Knockdown Validation miRNA Target Validation Promoter Activity Validation
  • Constitutive shRNA knockdown
  • Inducible shRNA knockdown
  • Validate the inhibitory effect of a particular miRNA on the 3'UTR region of GOI using luciferase reporter assays.
  • Detect exogenous promoter activation in mammalian cells using a luciferase reporter gene.

What are benefits of working with cell-based assays?

  • The biggest benefit of using cell-based assays is obvious: they better reflect the complexity of biological systems than simple biochemical assays.
  • Cell-based assays can be used to measure cellular behavior over time (up to months) and understand the impact of environmental factors.
  • Since cell-based assays can also be miniaturized and evaluated using multiplexing techniques, they are also increasingly used for high-throughput screening (HTS).
  • Cell-based assays save significant resources, time, and money not only in drug development.
About Us

Creative Bioarray is the world leading biological company whose mission focuses on the acquisition, authentication, production, and development of standard reference microbial strains, and cell lines.

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