cGMP BHK-21 Cell Banking

The BHK (Baby hamster kidney) cells, sort of fibrillogenic cells, are isolated from the kidney of an uninfected golden hamster and are characterized by continuous passaging. They can be used for the proliferation and purification of a variety of viruses, such as foot-and-mouth disease virus (FMDV), encephalomyocarditis virus (EMCV), vesicular stomatitis virus (VSV), cerebral myocarditis virus, and eutherian virus. Currently, the BHK-21 cell line has been widely used in the production of FMD vaccine.

Creating a cell bank is a vital element in producing consistent biological products. Cell banking systems are set up to assure that a uniform population of cells is preserved, their integrity is maintained, and a sufficient supply of material is accessible for the life of the product. Creative Bioarray provides a cGMP-compliant BHK-21 cell bank by following the industry-standard cell bank system to help our customers worldwide. ​Our experts will answer your questions comprehensively and carefully and help you solve the problems in your studies. We guarantee satisfied and reliable results for our customers all over the world.

Our Cell Services Cover the Complete Lifecycle of a Cell Bank

• Testing of developed cell lines BHK-21 prior to bank construction.
• Cell banking for master cell banks (MCB) under cGMP conditions. The establishment of a well-characterized cryopreserved master cell bank is a key step to assure the provision of a source of reliable cells for all future work.
• Cell banking for working cell banks (WCB) under cGMP conditions, which are usually necessary for subsequent stages of development and manufacturing. Vials from the MCB are recovered and expanded to prepare WCB from which individual vials are used to initiate cultures for each production run or period of experimentation.
• Complete validation and release of MCB and WCB in compliance with national regulatory requirements.
• Long-term secured storage services under cGMP management, including periodic storage reports, continuous environment monitoring, backup containers, etc.

Fig.1 Basic flow chart for the establishment and use of BHK-21 cell bank. (Radlett PJ, et al., 1985)

Advantages of Our BHK-21 Cell Banking Services

• Dedicated cGMP licensed facility.
• Usage of a two-tiered cell banking system.
• The performances of cell banks are assessed weekly against defined quality control procedures.
• A high viability cell bank for production, via a selection of reasonable freeze recovery time, higher viable cells, and appropriate cell concentration.
• Stable management measures for cell banking, including detailed cell documentation, stable culture conditions, and limited cell passaging.

Creative Bioarray provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.