| Tissue/Organ/Organ System | Adrenal Gland |
| Size | 1*106 cells/1.0 ml |
| Species | Human |
| Format | Frozen |
| Application | For Research Use Only |
| Cell Morphology | Polygonal |
| Description | H295RA is an ACTH-responsive human adrenocortical cell line, produced from H295R cells that have been transduced to stable express increased human MRAP (MC2R accessory protein) gene. H295R adrenocortical cell line displays relatively low ACTH responsiveness due to low melanocortin 2 receptor (MC2R) expression. Successful induction of MRAP and increased expression of MC2R in H295RA cells was confirmed by quantitative real-time RT-PCR and protein analysis. This cell line is well-suited for research regarding molecular analysis of physiologic and pathologic conditions involving the hypothalamic–pituitary–adrenal axis. |
| Quality Control | 1) Monoclonal selection using cloning cylinders; 2) quantitative real-time PCR; 3) Protein analysis |
| Donor Age | 48 years old |
| Donor Disease | Adrenocortical Carcinoma |
| Disease Subtype | Adrenocortical Carcinoma |
| Donor Ethnicity | African American |
| Donor Gender | Female |
| Population Doubling Time | 36 - 48 hours |
| Expression Profile | Transgenic expression of human MRAP; ACTH responsive steroid production and enzyme induction; angiotensin II responsive aldosterone production and aldosterone synthase expression. Selection marker: blasticidin 1ug/mL. Vector: pLX 304-Blast-V5 (Accession #BC062721) |
| Immortalization Method | Spontaneous |
| Price | Inquiry |
