| Tissue/Organ/Organ System | Esophagus/Stomach |
| Size | 1*106 cells/1.0 ml |
| Species | Human |
| Format | Frozen |
| Application | For Research Use Only |
| Cell Morphology | Epithelial-like, Polygonal |
| Description | The HGT-1 cell line was derived from a primary gastric adenocarcinoma tumor removed from a 60 year old Moroccan male of O Rh - blood group. At the time of the operation, the primary tumor showed no signs of metastasis but did exhibit poor differentiation. HGT-1 cells show hyperdiploid karyotype with a modal number of 57 chromosomes and are shown to be tumorgenic in nude mice. They also display functional histamine H 2 -receptors mediating cellular cyclic adenosine 3':5'-monophosphate production and adenylate cyclase activation. HGT-1 cells may have applications in the in vitro study of stomach cancer helping to identify the gene drives associated with gastric cancers. |
| Quality Control | 1) Calculated the growth curve of HGT-1 cells at passage 40 (Chart 1). 2) Observed morphology of HGT-1 cells at passage 70 3) Analyzed morphology of the primary tumor through microscopy (Figure 4). 4) Characterized the chromosomal markers and defects HGT-cells karyotype. (Table 1). 5) Tested the production of cyclic AMP and adenylate cyclase activity after the introduction of histamine alone or with cimetidine (Table 2) |
| Donor Age | 60 |
| Donor Disease | Esophagogastric Adenocarcinoma |
| Disease Subtype | Stomach Adenocarcinoma |
| Donor Ethnicity | Moroccan |
| Donor Gender | Male |
| Population Doubling Time | 19 hours |
| Price | Inquiry |
