Cat. No.: PCELL-1530

Human Endometrial Somatic Stem Cells (Stromal Fraction hESP-ICEs)

Description Human endometrial somatic stem cells are established from human endometrial side population cells
Tissue/Organ/Organ System Reproductive
Size 5*105 cells/1.0 ml
Species Human
Shipping Info Dry Ice
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Primary Cells
Applications For Research Use Only
Cell Morphology Fibroblast-like
Donor Disease Normal
Donor Gender Donor Info Not Disclosed
Expression Profile 1) Purity and origin of cells are confirmed by expression of CD9 (epithelial marker) or vimentin (stromal marker) and CD90, CD73 and CD105 (mesenchymal attributes); 2) Undifferentiated status of the cells are confirmed by expression of OCT4, NANOG, GDF3.
Isolation Method Endometrial Somatic Stem Cells are isolated based on the Side Population (SP) technique- a method that replies on the capability of cells to exclude the DNA binding Hoechst 33342 dye. Cells are labeled and sorted by flow cytometry based on low Hoechst 333.
Population Doubling Time Proliferation ceases after about 20 weeks of culture.
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