Cat. No.: ICELL-0103

Immortalized Human Adrenocortical Cells- hTERT

Description These cells can be used for research involving biochemical and molecular mechanisms related to adrenal steroid production and adrenal physiology.
Tissue/Organ/Organ System Renal
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1. Telomerase repeat amplification protocol (TRAP) assay was performed to measure telomerase activity in the immortalized cell line. 2) RT-PCR was used to quantify telomerase RNA component (TERC) expression in the immortalized cell line.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Cell Morphology Other
Immortalization Method Serial passaging and transduction with recombinant retroviruses carrying hTERT gene
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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