| Description | This cell line can be used not only to support the growth of hematopoietic cells, but also to study the bone marrow microenvironment and environmental clues regulating hematopoiesis. |
| Tissue/Organ/Organ System | Bone Marrow |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Immunofluorescence and RT-PCR were used to confirm the presence hTERT expression; 2) PCR based TRAP was used to assay telomerase activity; 3) Neo concentration not determined for selection use but cells were sorted for GFP+ populations |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 10,000 - 20,000 cells/cm2; Recommended split ratio is 1:2 to 1:3 |
| Applications | For Research Use Only |
| Cell Morphology | Spindle-shaped |
| Expression Profile | CD44, CD73; these cells also express markers of osteoblasts (alkaline phosphatise) and chondrocytes (incorporation of toluidine blue dye) |
| Immortalization Method | Serial passaging and transduction with recombinant VSV-G retrovirus carrying hTERT and GFP genes |
| Population Doubling Time | 35 - 45 hours |
| Price | Inquiry |
