Cat. No.: ICELL-0447

Immortalized Human Bronchial Epithelial Cells-hTERT/BMI

Tissue/Organ/Organ System Airway/Lung
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control Real-time PCR was used to quantify hTERT and BMI gene expression in immortalized cell lines.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 7,500 - 10,000 cells/cm2
Applications For Research Use Only
Cell Morphology Polygonal
Donor Age 55
Donor Gender Female
Expression Profile Cytokeratin
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying hTERT and BMI genes
Population Doubling Time ~23 hours
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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