Cat. No.: ICELL-0322

Immortalized Human Colonic Epithelial Cells (HCEC-1CT)

Description This cell line is a human colonic epithelial cell (HCEC) line isolated from a biopsy of a healthy adult patient
Tissue/Organ/Organ System Colon
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western blot; 2) Immunocytochemistry; 3) Transmission electron microscopy; 4) Karyotype and DNA sequence analysis
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Expression Profile LGR5, BMI1, CD29, and CD44In proliferation: vimentin, α-smooth muscle actinUpon growth arrest: cytokeratin 18, zonula occludens-1, mucins-1 and -2, antigen A33, and dipeptidyl peptidase 4
Immortalization Method Immortalized with retroviral Cdk4 and Human telomerase reverse transcriptase (hTERT)
Population Doubling Time 18 - 24 hours
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