Cat. No.: ICELL-0076

Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP)

Tissue/Organ/Organ System Reproductive
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Fibroblast-like
Donor Gender Female
Expression Profile CD90, CD34, CD44, CD45, CD166, CD117,CD73, CD105, CK18 and CK19
Immortalization Method Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145
Population Doubling Time Population doubling time of 23.8 ± 1.9 h
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