Tissue/Organ/Organ System |
Reproductive |
Size |
1*106 cells/1.0 ml |
Format |
Frozen |
Shipping Info |
Dry Ice |
Quality Control |
RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential. |
Storage Conditions |
Liquid Nitrogen |
Growth Properties |
Adherent |
Categories |
Immortalized Cells |
Applications |
For Research Use Only |
Cell Morphology |
Fibroblast-like |
Donor Gender |
Female |
Expression Profile |
CD90, CD34, CD44, CD45, CD166, CD117,CD73, CD105, CK18 and CK19 |
Immortalization Method |
Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145 |
Population Doubling Time |
Population doubling time of 23.8 ± 1.9 h |
Price |
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