| Description | The function of the corneal endothelium is to regulate corneal hydration. The dysfunction of this key cell layer will gradually lead to corneal opacity, eventually leading to vision loss and corneal blindness. |
| Tissue/Organ/Organ System | Eye |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7; 2) Indirect immunofluorescence was performed to detect the cell specific marker expression CX43, ZO-1 and 9.3E; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 20, 000 cells/cm2; Split ratio of 1:2 to 1:3 |
| Applications | For Research Use Only |
| Cell Morphology | Cobble-stone |
| Expression Profile | CX43, ZO-1, 9.3E |
| Immortalization Method | Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene |
| Population Doubling Time | 54 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
