Cat. No.: ICELL-0171

Immortalized Human Corneal Epithelial Cells (HCE-S)

Description Corneal epithelial cells are often difficult to maintain in an undifferentiated state in primary culture, and successful efforts to produce immortalized human lines are mainly limited to methods involving oncogenic transformation.
Tissue/Organ/Organ System Eye
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Scanning/transmission electron microscopy analysis; 2) Immunocytochemistry; 3) RT-PCR; 4) Western blot; 5) Scratch wound/proliferation/migration assay
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Epithelial
Donor Gender Female
Expression Profile Pancytokeratin, cytokeratin 3, ABCG2, α9 integrin, PAX 6, β1 integrin
Immortalization Method Spontaneous
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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