Description | Corneal epithelial cells are often difficult to maintain in an undifferentiated state in primary culture, and successful efforts to produce immortalized human lines are mainly limited to methods involving oncogenic transformation. |
Tissue/Organ/Organ System | Eye |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Scanning/transmission electron microscopy analysis; 2) Immunocytochemistry; 3) RT-PCR; 4) Western blot; 5) Scratch wound/proliferation/migration assay |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Applications | For Research Use Only |
Cell Morphology | Epithelial |
Donor Gender | Female |
Expression Profile | Pancytokeratin, cytokeratin 3, ABCG2, α9 integrin, PAX 6, β1 integrin |
Immortalization Method | Spontaneous |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |