| Description | The tissue repair and wound healing in the cornea are mainly completed by corneal fibroblasts, which are responsible for the pathological process of degenerative corneal diseases such as keratoconus. |
| Tissue/Organ/Organ System | Eye |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7; 2) Indirect immunofluorescence was performed to detect the cell specific marker expression CD36, ECM protein collagen I, and S100A4; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 10,000 - 20,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Spindle shaped |
| Expression Profile | CD36, ECM protein collagen I, S100A4 |
| Immortalization Method | Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene |
| Population Doubling Time | 70 - 80 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
