Tissue/Organ/Organ System | Brain |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | MRNA and protein expression levels of various markers pre and post differentiation are verified by RT-PCR and western blotting. |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Seeding Density | 50,000 - 100,000 cells/cm2; cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5 |
Applications | For Research Use Only |
Cell Morphology | Flattened|Dendritic processes |
Donor Age | 8 weeks old |
Expression Profile | DAT, VMAT-2, TH, α-SYN, β-III tubulin |
Immortalization Method | Conditional immortalization by tetracyclin-controlled transduction with retrovirus carrying v-myc genes |
Population Doubling Time | 30 - 40 hours |
Price | Inquiry |