Cat. No.: ICELL-0285

Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES)

Tissue/Organ/Organ System Brain
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control MRNA and protein expression levels of various markers pre and post differentiation are verified by RT-PCR and western blotting.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 50,000 - 100,000 cells/cm2; cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5
Applications For Research Use Only
Cell Morphology Flattened|Dendritic processes
Donor Age 8 weeks old
Expression Profile DAT, VMAT-2, TH, α-SYN, β-III tubulin
Immortalization Method Conditional immortalization by tetracyclin-controlled transduction with retrovirus carrying v-myc genes
Population Doubling Time 30 - 40 hours
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