Cat. No.: ICELL-0073

Immortalized Human Endometrial Stromal Cells (HESC)

Description The cells are collected from the endometrium of women undergoing hysterectomy, so there is no specific age.
Tissue/Organ/Organ System Reproductive
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 10,000 – 20,000 cells/cm2 Recommended split ratio of 1:3 to 1:6
Applications For Research Use Only
Cell Morphology Fibroblast-like
Donor Age Adult
Donor Disease Non-malignant myomas (Fibroids).
Donor Gender Female
Expression Profile IGFBP-1, PRL, TF, PAI-1 at decidualization endpoints
Immortalization Method Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line
Population Doubling Time 32-42 hours
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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