Cat. No.: ICELL-0201

Immortalized Human Fetal Liver Cells (cBAL111)

Description Human fetal hematopoietic cells are immortalized by stable lentiviral overexpression of human telomerase reverse transcriptase (hTERT).
Tissue/Organ/Organ System Liver
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) qRT-PCR for hTERT expression; 2) cBAL111 clone continued to proliferate whereas primary cells senescenced in culture; 3) Immunostaining conducted showed cells are positive for hepatic markers albumin, glutamin synthetase and cytokeratin 18, and positive for cholangiocyte marker cytokeratin 19.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Spindle-shaped
Expression Profile Ampicillin & neomycine-resistant, expresses albumin, glutamin synthetase, cytokeratin 18, and cytokeratin 19.
Immortalization Method Immortalized with lentivirus containing hTERT
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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