Description | Human fetal hematopoietic cells are immortalized by stable lentiviral overexpression of human telomerase reverse transcriptase (hTERT). |
Tissue/Organ/Organ System | Liver |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) qRT-PCR for hTERT expression; 2) cBAL111 clone continued to proliferate whereas primary cells senescenced in culture; 3) Immunostaining conducted showed cells are positive for hepatic markers albumin, glutamin synthetase and cytokeratin 18, and positive for cholangiocyte marker cytokeratin 19. |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Applications | For Research Use Only |
Cell Morphology | Spindle-shaped |
Expression Profile | Ampicillin & neomycine-resistant, expresses albumin, glutamin synthetase, cytokeratin 18, and cytokeratin 19. |
Immortalization Method | Immortalized with lentivirus containing hTERT |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |