| Description | Gingival fibroblasts play an important role in oral wound healing and are the main component of gingival connective tissue. |
| Tissue/Organ/Organ System | Oral |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1. Immunofluorescence, RT-PCR and Western Blotting were used to confirm the presence of hTERT expression (PCR primers for hTERT: 5’-CGGAAGAGTGTCTGGAGCAA-3’ forward, and 5’-GGATGAAGCGGAGTCTGGA-3’ reverse). 2. MTT assay was used to confirm the proliferative potential of the immortalized cells over the 70th passage. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 7,000 - 10,000 cells/cm2; Recommended split ratio is 1:2 to 1:3 |
| Applications | For research only. The Immortalized Human Gingival Fibroblast -hTERT is unique that 1. The cells retained similar cytotoxicity response pattern to their primary counterparts (in response to phenol, gold, titanium and amalgam); 2. The cells retained identical phenotype to that of their primary counterparts; 3. The cells have a diploid status, showing genomic stability. |
| Cell Morphology | Spindle shaped |
| Expression Profile | Vimentin and actin |
| Immortalization Method | Serial passaging and transduction with recombinant retroviruses carrying hTERT gene |
| Population Doubling Time | 50 - 60 hours |
| Price | Inquiry |
