Cat. No.: ICELL-0189

Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc

Description DPC is a useful tool for studying the regulation of hair growth.
Tissue/Organ/Organ System Hair Follicle
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery).
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 20,000 - 30,000 cells/cm2, Recommended Split ratio of 1:2
Applications For Research Use Only
Cell Morphology Flat|Polygonal
Donor Age Donor Info Not Disclosed
Donor Disease Donor Info Not Disclosed
Donor Ethnicity Donor Info Not Disclosed
Donor Gender Donor Info Not Disclosed
Expression Profile Smooth muscle alpha actin, alkaline phosphatase
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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