Description | DPC is a useful tool for studying the regulation of hair growth. |
Tissue/Organ/Organ System | Hair Follicle |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery). |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Seeding Density | 20,000 - 30,000 cells/cm2, Recommended Split ratio of 1:2 |
Applications | For Research Use Only |
Cell Morphology | Flat|Polygonal |
Donor Age | Donor Info Not Disclosed |
Donor Disease | Donor Info Not Disclosed |
Donor Ethnicity | Donor Info Not Disclosed |
Donor Gender | Donor Info Not Disclosed |
Expression Profile | Smooth muscle alpha actin, alkaline phosphatase |
Immortalization Method | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |