| Description | Sinusoidal endothelial cells are microvascular endothelial cells with a phenotype similar to dendritic cells and a unique function as CD4+ T cell antigen presenting cells. |
| Tissue/Organ/Organ System | Liver |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | Real Time PCR was used to quantify SV40 gene expression in immortalized cell line |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 20,000 cells/cm2; Recommended split ratio is 1:3 to 1:4 |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Donor Age | Adult |
| Expression Profile | CK18, CK19, vWF, CD31, VE-cadherin, puromycin resistance |
| Immortalization Method | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen |
| Population Doubling Time | 12 - 22 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
