Cat. No.: ICELL-0325

Immortalized Human Laryngeal Posterior Commissure Cells

Description The posterior commissural area is composed of cells that help to make sounds.
Tissue/Organ/Organ System Digestive
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 20,000 - 30,000 cells per cm2.
Applications For Research Use Only
Cell Morphology Epithelial (<10% fibroblasts)
Expression Profile CK5, CK8
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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