Description |
Immortalized human breast epithelial cells are an immortalized breast epithelial cell line that is not tumorigenic and does not show anchorage-independent growth. |
Tissue/Organ/Organ System |
Breast |
Size |
1*106 cells/1.0 ml |
Format |
Frozen |
Shipping Info |
Dry Ice |
Quality Control |
1) Cell markers were probed via immunohistochemical analysis; 2) Soft agar assay preformed to determine anchorage-independence |
Storage Conditions |
Liquid Nitrogen |
Growth Properties |
Adherent |
Categories |
Immortalized Cells |
Seeding Density |
10,000 - 15,000 cells/cm2 |
Applications |
For Research Use Only |
Cell Morphology |
Polygonal |
Donor Gender |
Female |
Expression Profile |
E-cadherin, EGFR, MIB1, CD10, CK5/14, P63, and CK7/18 |
Immortalization Method |
Serial passaging and transduction with LXSN-based retroviruses carrying hTERT and a mutant CDK4(R24C) |
Population Doubling Time |
Mean doubling time of around 72 hours |
Price |
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