Description | Immortalized human breast progenitor cells-hTERT (K5+/K19+) is a clonal progenitor cell population that co-expresses normal breast and stem cell markers. |
Tissue/Organ/Organ System | Breast |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Western blot to assess cell lineage-related and stem cell markers; 2) Immunofluorescence staining analysis for lineage markers; 3) Gene expression profiling for gene regulatory pathways |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Applications | For Research Use Only |
Cell Morphology | Cobble-stone|Spindle shaped |
Expression Profile | K5, K14, vimentin, E-cadherin, p63, K8, K18, K19, CD29, CD49f a-SMA, CD10, Thy-1 when grown in DCFI-1 |
Immortalization Method | Serial passaging and transduction with recombinant retroviruses carrying hTERT gene |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |