Cat. No.: ICELL-0309

Immortalized Human Mammary Epithelial Progenitor (K5+/K19+) Cells - hTERT

Description Immortalized human breast progenitor cells-hTERT (K5+/K19+) is a clonal progenitor cell population that co-expresses normal breast and stem cell markers.
Tissue/Organ/Organ System Breast
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western blot to assess cell lineage-related and stem cell markers; 2) Immunofluorescence staining analysis for lineage markers; 3) Gene expression profiling for gene regulatory pathways
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Cobble-stone|Spindle shaped
Expression Profile K5, K14, vimentin, E-cadherin, p63, K8, K18, K19, CD29, CD49f a-SMA, CD10, Thy-1 when grown in DCFI-1
Immortalization Method Serial passaging and transduction with recombinant retroviruses carrying hTERT gene
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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