| Description | Immortalized human mammary fibroblasts (HMF3A) are produced by the combined transduction of mammary fibroblasts with temperature-sensitive mutant SV40 large T antigen (U19tsA58) and human telomerase catalytic subunit (hTERT). |
| Tissue/Organ/Organ System | Mammary |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Telomerase-detection assay was used to detect telomerase activity. 2) Flow cytometry confirmed expression of T antigen. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 10,000 - 20,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Spindle shaped |
| Immortalization Method | Combined transduction with retrovirus carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT) |
| Population Doubling Time | 57 - 67 hours |
| Price | Inquiry |
