Description | HMF3S cells grow best at 37⁰C and are insensitive to temperature compared to immortalized human mammary fibroblasts (HMF3A) |
Tissue/Organ/Organ System | Mammary |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Telomerase-detection assay was used to detect telomerase activity. 2) Flow cytometry confirmed expression of T antigen. |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Seeding Density | 10,000 - 15,000 cells/cm2 |
Applications | For Research Use Only |
Cell Morphology | Spindle shaped |
Immortalization Method | Combined transduction with retroviruses carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT) |
Population Doubling Time | 45 hours |
Price | Inquiry |