Description | Immortalized marmoset liver progenitor cells (MHPC) are produced by lentivirus-mediated transfer of the 40 large T antigen genes of monkey virus into fetal liver polygonal cells. |
Tissue/Organ/Organ System | Liver |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Karyotype and telomere analysis; 2) RNA sequencing; 3) Reverse transcription-PCR; 4) Immunocytochemistry and immunohistochemistry; 5) Histology; 6) PAS, ICG, and CDFDA uptake assays and Oil Red O staining; 7) In vitro differentiation; 8) CYP450 induction |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Applications | For Research Use Only |
Cell Morphology | Elongated |
Expression Profile | Hepatic progenitor markers (CD44, Sox9, Sox17 and Ncam1) |
Immortalization Method | Immortalized with lentivirus containing the SV40 T antigen |
Population Doubling Time | 55 hours |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |