| Description | Immortalized mouse articular chondrocytes are hygromycin-resistant, non-tumorigenic and reversible immortalized cells, retaining the morphological and chondrogenic characteristics. |
| Tissue/Organ/Organ System | Skeletal |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | RT-PCR, qPCR, sqPCR analysis for mouse gene of interest; 2) Col 1al immunohistochemical staining preformed; 3) Western blot used to detect SV40 large T antigen presence; 4) Alcian blue staining analysis for chondroid matrix production. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 20,000 - 40,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Immortalization Method | Serial passaging and infection with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites |
| Population Doubling Time | 40 - 50 hours |
| Price | Inquiry |
