Cat. No.: ICELL-0178

Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM)

Description Compared with parental cells, immortalized cells show the same characteristics.
Tissue/Organ/Organ System Heart
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Immunostaining analysis; 2) Microarray analysis; 3) RT-PCR analysis;
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Polygonal
Expression Profile CTnt, MHC, PECAM, NFACT1, SMA, Vimentin
Immortalization Method Conditional immortalization achieved in subsequent cultures as temperature sensitive SV40T antigen mutant gene (tsA58) expression driven by the c-interferon inducible promoter of the H-2Kb gene.
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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