Cat. No.: ICELL-0157

Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1))

Tissue/Organ/Organ System Kidney
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Immunofluorescence and RT-PCR were used to confirm the expression of epithelial sodium channel (ENaC) and Na-K-ATPase; 2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2 ; 3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density In early passages, the cells do not form domes and grow more slowly. Split low passage cells at 1:6 ratio. At higher passages (above p15), the cells grow more rapidly and are recommended to be split at 1:10 ratio.
Applications For Research Use Only
Cell Morphology Other
Expression Profile Cytokeratin 18, Cytokeratin 19
Immortalization Method Spontaneously immortalization
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