| Description | The immortalized mouse cortical collection tube cell line is a spontaneously transformed Liddle mouse cell line that can express significant levels of functionally active MR and GR, as well as active 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2) enzymes |
| Tissue/Organ/Organ System | Kidney |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Immunofluorescence and RT-PCR were used to confirm the expression of epithelial sodium channel (ENaC) and Na-K-ATPase; 2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2 ; 3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | In early passages, the cells do not form domes and grow more slowly. Split low passage cells at 1:6 ratio. At higher passages (above p15), the cells grow more rapidly and are recommended to be split at 1:10 ratio. |
| Applications | For Research Use Only |
| Cell Morphology | Other |
| Expression Profile | Cytokeratin 18, Cytokeratin 19 |
| Immortalization Method | Spontaneously immortalization |
| Price | Inquiry |
