Cat. No.: ICELL-0265

Immortalized Mouse Dental Apical Papilla Progenitor Cells (iSCAP)

Description It is recommended that these cells be used in research involving apical papilla stem cell biology and dental tissue engineering.
Tissue/Organ/Organ System Oral
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Immunofluorescence staining performed to examine mesenchymal stem cell markers; 2) sqPCR analysis for BMP9-induced late osteo/odontoblast, and ondotoblast markers.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Cell Morphology Polygonal
Expression Profile CD73, CD44, CD90/Thy-1, CD105/Endoglin, CD166/ALCAM, CD133/Prom1, BMPRII, CD117/c-kit, and CD29/integrin β1
Immortalization Method Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
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