| Description | Sinusoidal endothelial cells (HSEC) are a unique subgroup of hepatocytes that line the sinusoids. |
| Tissue/Organ/Organ System | Liver |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1. Real-time fluorescent quantitative PCR is used to quantify the expression of SV40 gene in immortalized cell lines. 2. Use Western Blot to confirm the presence of endothelial markers, such as vWF and caveolin-1. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 5,000 - 7,000 cells/cm2; Recommended split ratio is 1:2 to 1:3 |
| Applications | For research only. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is unique in its 1. Ability to endocytose AcLDL; 2. Increased migration rate to VEGF and FGF; 3. Ability to form robust vascular tube-like structures; 4. 90% transduction efficiency using retrovirus and 5. Tolerance to serum starvation. |
| Cell Morphology | Polygonal |
| Expression Profile | VWF and caveolin-1 |
| Immortalization Method | Serial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigen |
| Population Doubling Time | 22 - 32 hours |
| Price | Inquiry |
