Cat. No.: ICELL-0382

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

Description ImHep is a conditionally immortalized mouse hepatocyte, isolated from mice carrying a heat labile mutation (tsA58), and is affected by interferon (IFN)-γ-inducible H-2Kb promoter and temperature-sensitive simian virus 40 big T antigen control.
Tissue/Organ/Organ System Liver
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western Blot and immunofluorescence method to detect hepatocyte markers albumin, AFP, transferrin, LPK, CK8 and CK18; 2) immunofluorescence method to verify the presence of SV40 large T antigen
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Recommended split ratio of 1:3
Applications For Research Use Only
Cell Morphology Other
Expression Profile Albumin, AFP, transferrin, LPK, CK8 and CK18
Immortalization Method Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen
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