Cat. No.: ICELL-0304

Immortalized Mouse Mammary Epithelial Cells (iMMEC)

Description This cell line is suitable for the study of hormone regulation and 3D stereo duct morphogenesis.
Tissue/Organ/Organ System Breast
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western blot; 2) Immunofluorescence
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Seeding Density 25,000 - 40,000 cells/cm2
Applications For Research Use Only
Cell Morphology Cuboidal
Donor Age 6- to 8-weeks old
Donor Gender Female
Expression Profile β-catenin, E-cadherin, Ep-CAM, CK8 and CK18; note that these cells lack drug-resistance markers
Immortalization Method Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Population Doubling Time 40 - 50 hours
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