Cat. No.: ICELL-0381

Immortalized Mouse MyD88-/- Hepatic Stellate Cells

Description Immortalized mouse hepatic stellate cells were derived from MyD88-/- mice and were digested with enzymes and centrifuged with Percoll density gradients.
Tissue/Organ/Organ System Liver
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) 3[H]-thymidine Cell Proliferation Assay
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density 10,000 cells/cm2
Applications For Research Use Only
Cell Morphology Polygonal
Immortalization Method PCMV Simian Virus 40 Large T antigen
Population Doubling Time 25-35 hours
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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