Description | Immortalized mouse hepatic stellate cells were derived from MyD88-/- mice and were digested with enzymes and centrifuged with Percoll density gradients. |
Tissue/Organ/Organ System | Liver |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) 3[H]-thymidine Cell Proliferation Assay |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Seeding Density | 10,000 cells/cm2 |
Applications | For Research Use Only |
Cell Morphology | Polygonal |
Immortalization Method | PCMV Simian Virus 40 Large T antigen |
Population Doubling Time | 25-35 hours |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |