| Description | IDG-SW3 represents a heterogeneous population that develops from early osteoblasts to late osteocytes. |
| Tissue/Organ/Organ System | Skeletal |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) The presence and absence of SV40 big T antigen are confirmed by Western blot under permitted and non-permitted conditions. 2) Fluorescence imaging is used to measure the expression of GFP driven by the Dmp1 promoter under osteogenic conditions. 3) Von Kossa staining showed focal nodule mineralization, Alizarin Red S staining detected calcium deposition in differentiated cells. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 25,000 - 30,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Expression Profile | ALP (osteoblast), E11/gp38, Dmp1 and Phex (osteocyte), SOST/sclerostin and FGF23 (late osteocyte) |
| Immortalization Method | Isolated from long bones of transgenic mice (in which the Dmp1 promoter drives the expression of GFP). |
| Population Doubling Time | 40 - 50 hours |
| Price | Inquiry |
