Cat. No.: ICELL-0145

Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)

Description IMPE has been shown to produce insulin after being transfected with pdx1 and cultured in the presence of glucagon-like peptide 1.
Tissue/Organ/Organ System Pancreas
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Western Blot and immunofluorescence were used to detect the presence of SV40 large T antigen; 2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin; 3) RT-PCR was used to verify the expression of CK8, CK18 and CK19.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Recommended split ratio of 1:3
Applications For Research Use Only
Cell Morphology Cobble-stone
Expression Profile β-catenin, E-Cadherin, CK8, CK18, CK19
Immortalization Method Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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