Description | IMPE has been shown to produce insulin after being transfected with pdx1 and cultured in the presence of glucagon-like peptide 1. |
Tissue/Organ/Organ System | Pancreas |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Western Blot and immunofluorescence were used to detect the presence of SV40 large T antigen; 2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin; 3) RT-PCR was used to verify the expression of CK8, CK18 and CK19. |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Seeding Density | Recommended split ratio of 1:3 |
Applications | For Research Use Only |
Cell Morphology | Cobble-stone |
Expression Profile | β-catenin, E-Cadherin, CK8, CK18, CK19 |
Immortalization Method | Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58) |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |