| Description | Immortalized mouse retinal pigment epithelial cells-HPV E6/E7, derived from healthy C57B1/6 mouse RPE cells, is a cell line that retains many in vivo phenotypic characteristics. |
| Tissue/Organ/Organ System | Eye |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1. Western Blot was used to verify the HPV protein expression in the immortalized cell line. 2. Western blot and real-time RT-PCR was used to confirm the presence of in vivo RPE markers such as RPE65 and CRALBP. 3. Immunofluorescence staining was used to confirm expression of epithelial cell markers ZO1, cytokeratin 8 and cytokeratin 18. 4. Absence of neural cell marker, SV2, PKCα, rhodopsin |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 5,000 - 10,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Cobble-stone |
| Expression Profile | RPE65, CRALBP, ZO1, Cytokeratin -8 and -18 |
| Immortalization Method | Serial passaging and transduction with retrovirus carrying human papiloma virus 16 E6/E7 |
| Population Doubling Time | ~50 hours |
| Price | Inquiry |
