Cat. No.: ICELL-0283

Immortalized Mouse Stomach Epithelial Cells-Conditionally (ImSt)

Description ImSt is a conditionally immortalized mouse gastric epithelial cell line, isolated from mice carrying a heat-labile mutation (tsA58), and is affected by interferon (IFN)-γ-inducible H-2Kb promoter and temperature-sensitive Control of 40 big T antigen of simian virus.
Tissue/Organ/Organ System Digestive
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control Immunohistochemical techniques were utilized detect keratin 18 and E-cadherin to demonstrate the epithelial nature of cells.
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Seeding Density Recommended split ratio of 1:3
Applications For Research Use Only
Cell Morphology Other
Expression Profile CK18
Immortalization Method Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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