| Description | PAMs were isolated from ten 10-week-old clinically healthy Yorkshire pigs raised at a local pig farm. Primary porcine alveolar macrophages (PAM) are useful for studying viral infections and immune response in pigs. Immortalized PAM cells possessed the characteristics of primary PAMs including strong expression of swine leukocyte antigen (SLA) class II genes and the inability to grow anchorage-independently. iPAM303 (73.3%+-5.4; SLA DQA*0106/0201; DQB1*0202/0701; DRB1*0402/0602). |
| Tissue/Organ/Organ System | Lung |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Cell Morphology | Fibroblast-like |
| Donor Age | 10 weeks old |
| Expression Profile | Puromycin resistance |
| Immortalization Method | Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
