| Description | Immortalized porcine aortic endothelial cells (AOC) are derived from the stable transformation of the SV40 genome into primary porcine aortic endothelial cells. |
| Tissue/Organ/Organ System | Blood Vessel |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens; 2) 51Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity; 3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI)-labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 25,000-30,000 cells/cm2; Recommended split ratio is 1:2 to 1:3 |
| Applications | For Research Use Only |
| Cell Morphology | Cobble-stone |
| Expression Profile | CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 |
| Immortalization Method | Tranfection with pRNS-1 plasmid encoding the SV40 genome |
| Population Doubling Time | 50-60 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
