Description | Immortalized rainbow trout heart cells (RTH) are produced by transducing rainbow trout heart with a recombinant retrovirus carrying polyoma intermediate T antigen. |
Tissue/Organ/Organ System | Heart |
Size | 1*106 cells/1.0 ml |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) Evaluation of immune gene expression by real time PCR; 2) Phagocytosis assay; 3) Viral infection of RTH cells with VHSV |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Immortalized Cells |
Applications | For Research Use Only |
Cell Morphology | Cobblestone |
Expression Profile | Constitutive transcription of collagen, selectin, VCAM, CK9, CK10, CK11, CK12, CXCL11_L1, CXCL_F1, CCR7, CXCR1, CXCR3B, and CXCR4 |
Immortalization Method | Immortalized with recombinant retroviruses containing polyoma middle T antigen |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |