Cat. No.: ICELL-0392

Immortalized Rainbow Trout Heart Cells (RTH)

Description Immortalized rainbow trout heart cells (RTH) are produced by transducing rainbow trout heart with a recombinant retrovirus carrying polyoma intermediate T antigen.
Tissue/Organ/Organ System Heart
Size 1*106 cells/1.0 ml
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Evaluation of immune gene expression by real time PCR; 2) Phagocytosis assay; 3) Viral infection of RTH cells with VHSV
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Immortalized Cells
Applications For Research Use Only
Cell Morphology Cobblestone
Expression Profile Constitutive transcription of collagen, selectin, VCAM, CK9, CK10, CK11, CK12, CXCL11_L1, CXCL_F1, CCR7, CXCR1, CXCR3B, and CXCR4
Immortalization Method Immortalized with recombinant retroviruses containing polyoma middle T antigen
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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