Insect Cell Bank Characterization

With the development of life science, cell engineering has been increasingly emphasized in the field of bioengineering technology. Insect cell culture technology with insect cells as the object of culture is of great value in modern experimental biology and is widely used in various fields of medicine, agriculture, and biology. The establishment of insect cell banks provides the necessary technical and material support for these applications, and cell bank characterization is important for safe and controlled biotechnological products.

Creative Bioarray provides comprehensive and detailed services for insect cell bank characterization to help our customers worldwide in the field of cell banks, which are well characterized and have been tested covering master cell banks (MCB), working cell banks (WCB), end-of-production cell banks (EPC) and cells at the limit (CAL). This ensures the identity, purity, and safety of the cells and the resulting biological product. Rapid and accurate results will be delivered to assist customers in accessing the quality of cell banks as soon as possible.

Our Cell Bank Characterization Services Cover the Following Aspects

Detecting microbial and viral contaminants

• Bacterial testing and virus testing, including but not limited to in vitro / in vivo adventitious virus testing.
• Mycoplasma testing. In the case of mycoplasma, several tests are available. These include growth assays using mycoplasma culture medium, screening with fluorescent nuclear dyes, or coculture with 6-methylpurine which is metabolized by mycoplasma to form toxic components.
• Sterility testing. This testing is conducted by direct inoculation methods and can be performed in an isolator or cleanroom environment.

Verifying cell line identity

• Karyology testing, but lepidopteran insects are one of the exceptions. Most cell lines from Lepidoptera are highly polyploid and made up of small chromosomes which are impossible to properly karyotype.

• Isoenzyme analysis. The isoenzyme technique has been analyzed for use with insect cells, while organisms have many shared enzyme systems, the particular enzyme protein from a specific organism may differ from other, even closely related, organisms. These differences can be discerned through electrophoretic techniques.
• DNA fingerprinting. It includes four phases, sample collection, DNA extraction, restriction uptake, enhancement and DNA sequencing, and analysis of results.
• Genetic profiling (STR).

Confirming genetic stability

• Gene copy number by QPCR.
• Restriction enzyme analysis by Southern Blot.
• Expression cassette sequencing.
• Fluorescence in situ hybridization (FISH).
• Retention of the selectable marker.
• Retention of the recombinant construct.
• DNA / RNA sequencing.

Others

Tumorigenicity testing in nude mice.

As a pioneer in biosafety testing service, Creative Bioarray provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We offer international testing methodologies for insect cell bank characterization. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.