Cat. No.: SCELL-00005

PIK3CA_x001f_ (E545K/+) MCF10A Cell Line

Description The Cell line from CD BioSciences is Heterozygous knockin of PIK3CA helix domain activating mutation.
Size/Quantity 1 vial
Species Human
Biosafety Level 1
Shipping Info Dry Ice
Storage Liquid Nitrogen
Quality Control All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Recommended Medium And Supplement Growth Medium: DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 5% horse serum, 10 µg/mL RI, 0.5 µg/mL hydrocortisone, 0.1 µg/mL cholera toxin.; Freeze Medium: Complete growth media 45%; DMSO 5%; Reviv
Growth Properties The cells are cultured as a monolayer in a humid environment at 37°C and 5% CO2. Cells should be passaged every 3-5 days.
Application For research use only
Stock Knockin Stable Cell Line
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