Process Development for Protein Conjugates

Protein conjugates can be used for a variety of applications including protein blotting, ELISA, and immunotoxins for drug delivery. In this case, the physicochemical properties and functions of the protein are extended. This is usually achieved by conjugation reactions, where additional molecules are attached to the protein. A common example of an additional molecule is a polymer chain, such as polyethylene glycol (PEG), which is widely used to increase the in vivo circulating half-life of a drug. In addition to PEG, other polymers such as N-(2-hydroxypropyl) meth acrylamide, polyglutamic acid, dextrin, hydroxyethyl starch, or polysilicicic acid can also be employed.

Fig. 1 Common protein conjugates. (David P, et al., 2018)

Creative Bioarray makes full use of our specialized libraries, selection strategies, and maturation techniques to provide high-quality services to global clients. With advanced technology and experienced staff, we provide comprehensive cell support services in the process development of protein conjugates.

Our Processing of Protein Conjugates

• Protein manufacturing.
• Phenolic-protein conjugates process. Synthetic methods of phenolic-protein conjugates. We provide three main methods, including the alkaline method, free radical-mediated grafting method, and chemical coupling method. Among them, the alkaline method is a simple and effective method to synthesize phenolic-protein conjugates and phenolic compounds can be easily conjugated with proteins by chemical coupling reagents.
• Polymer-protein conjugates process. Synthetic methods of phenolic-protein conjugates. We provide three main strategies, including ligation chemistry in the 'grafting onto' approach, random versus site-specific coupling, the 'grafting from' approach, and the multi-point covalent boding approach.
• Cyclodextrins-protein conjugates process. The cyclodextrins (CDs)-protein conjugates can possess new abilities; particularly, the cavity can be applied in the modulation properties of more complex proteins. Most studies are focused on drug delivery, such as targeted delivery in cell-penetrating peptides or co-delivery. on the other hand, the CD-peptide allows us to understand biomolecular mechanisms such as fibrillation or stem cell behavior.

The Role of Cell Banks in Protein Manufacturing

• Most of the therapeutic proteins are manufactured from engineered mammalian cell lines. Mammalian systems are often preferred over other hosts, such as bacteria, plants, and yeast, because of their capability for proper protein folding, assembly, and posttranslational modification.
• We offer high-quality cGMP-compliant CHO, NS0, Sp2/0, and other cell banking services as strong support for protein manufacturing in the protein conjugates processing, which is a cost-efficient alternative to keeping cells in a culture constantly.

As a professional service provider in the field of cell science, Creative Bioarray provides strong cell support in the process development for various protein conjugates to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.