Therapeutics oligonucleotides (ONs) are state-of-the-art, molecular-target agents that employ chemically synthesized oligonucleotides with a single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) backbone with potential specificity. These agents can inhibit gene expression or impede protein function by binding to a specific sequence of a target gene or protein. Antisense gamer, steric block, splice-switching ONs, and short-interfering RNA drugs have been successfully developed. Moreover, antagomirs (anti-microRNAs), microRNA mimics, aptamers, DNA decoys, DNAzymes, synthetic guide strands for CRISPR/Cas, and innate immunity-stimulating ONs are all in clinical trials.
With advanced technology and experienced staff, Creative Bioarray provides comprehensive cell support services in the process development for therapeutic oligonucleotides to help our customers worldwide. We delivered rapid and accurate results to assist customers to accelerate their research.
Fig. 1 Manufacturing process of therapeutic oligonucleotides.
| Solid phase synthesis | During solid-phase synthesis, phosphoramidite monomers are added sequentially onto a solid support to generate the desired full-length oligonucleotide. Each cycle of base addition consists of four chemical reactions, deacetylation, coupling, oxidation/thiolation, and capping. |
| Cleavage and deprotection (C&D) | Once the synthesis sequence is complete, oligos are cleaved from the solid support. After cleavage, the solution of the oligonucleotide is heated in concentrated aqueous ammonium hydroxide to eliminate protecting groups from the bases and phosphates. Deprotection then removes blocks that are used to ensure linear chains versus split chains. |
| Purification | The purification of the oligonucleotide crude solution is usually achieved by chromatographic methods. |
| Concentration | Tangential flow filtration is utilized for therapeutic oligonucleotide isolation at manufacturing scales. |
| Lyophilization | The most common current practice is to lyophilize (freeze-dry) the drug substance before formulation. |
As a professional services provider in the field of cell science, Creative Bioarray provides high-quality cell support services in the process of development for therapeutic oligonucleotides to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.
Reference
Creative Bioarray is the world leading biological company whose mission focuses on the acquisition, authentication, production, and development of standard reference microbial strains, and cell lines.
