Description | Human Epidermal Keratinocytes (HEK) provide an excellent model for studying epithelial function and disease, skin biology, toxicology, and HPV infection. When growing on the insert and providing a liquid/air interface, they differentiate into layers of the squamous epithelium to form physiological 3D tissue. HEK constitutes 90% of all cells in the epidermis (the outermost skin layer). They create a barrier, intermediate filament keratin. Keratinocytes obtain melanin from melanocytes (HEM) and store it to protect against ultraviolet radiation. We strongly recommend the use of an optimized Human EpiVita medium to enhance the performance and in vitro life of HEK. |
Size/Quantity | T-25 Flask/T-75 Flask/24 well/96 Well |
Quality Control | No bacteria, yeast, fungi, mycoplasma, virus |
Application | For research use only |
Tissue | Normal healthy human foreskin or adult skin |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HEK (Primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial HEK (102-05), Epi-Vita Growth Medium (141-500a), Subcltr Rgnt Kit (090K) |
Proliferating | In Serum-Fr Gr Med, psg 1, flasks or plates |
Doublings | At least 16 in keratinocyte media |
Price | Inquiry |