Cat. No.: ICELL-1050

Stable MEF Raf1 FF/FF Knockin Cell Line

Description Stable MEF Raf1 FF/FF knock-in cells were obtained from knock-in mouse embryos carrying inactive Raf-1FF/FF mutants.
Tissue/Organ/Organ System Embryo
Size 1*106 cells/1.0 ml
Species Mouse
Format Frozen
Shipping Info Dry Ice
Quality Control 1) FACS; 2)Western blot; 3) Raf kinase cascade assay; 4) MBP kinase assay
Storage Conditions Liquid Nitrogen
Growth Properties Adherent
Categories Drug Discovery Cell Lines
Seeding Density 20,000 - 40,000 cells/cm2
Applications For Research Use Only
Cell Morphology Fibroblast-like
Donor Age Embryonic
Donor Gender Female
Expression Profile Raf1 Y340F Y341F inactive mutant. Vector: pZIPTEX; neomycin resistance
Preservation Protocol 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid Nitrogen vapour phase.
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