Description | Stable MEF Raf1 FF/FF knock-in cells were obtained from knock-in mouse embryos carrying inactive Raf-1FF/FF mutants. |
Tissue/Organ/Organ System | Embryo |
Size | 1*106 cells/1.0 ml |
Species | Mouse |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) FACS; 2)Western blot; 3) Raf kinase cascade assay; 4) MBP kinase assay |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Drug Discovery Cell Lines |
Seeding Density | 20,000 - 40,000 cells/cm2 |
Applications | For Research Use Only |
Cell Morphology | Fibroblast-like |
Donor Age | Embryonic |
Donor Gender | Female |
Expression Profile | Raf1 Y340F Y341F inactive mutant. Vector: pZIPTEX; neomycin resistance |
Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
Price | Inquiry |