Description | Using CRISPR technology, Mgat2 was silenced from K16 CHO cells to generate a glycosylation mutant cell line expressing heterozygous N-glycans. |
Tissue/Organ/Organ System | Reproductive |
Size | 1*106 cells/1.0 ml |
Species | Golden Hamster |
Format | Frozen |
Shipping Info | Dry Ice |
Quality Control | 1) 4 µg/ml of puromycin (G264) was used for clonal selection; 2) Mgat2 silencing was confirmed by DNA sequencing of targeted genomic region; 3) E-cadherin was determined through the use of Western blot method |
Storage Conditions | Liquid Nitrogen |
Growth Properties | Adherent |
Categories | Drug Discovery Cells |
Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
Applications | For Research Use Only |
Cell Morphology | Polygonal |
Expression Profile | E-cadherin |
Pharmaceutical Target | Enzymes |
Price | Inquiry |