Cat. No.: ICELL-0658

Stable ΔN-IκBα Expressing HOS-G (HOS-G/ΔN-IκBα) Cell Line

Description It is suggested that this cell line can be used to study HTLV-1 replication in cell culture.
Tissue/Organ/Organ System Skeletal
Size 1*106 cells/1.0 ml
Species Human
Format Frozen
Shipping Info Dry Ice
Quality Control 1) Verify the expression of GFP after HTLV-1 infection by imaging 2) Verify the expression of δN-IκBα in Western blot 3) Verify that the cell line continues to multiply after HTLV-1 infection 4) Confirm the expression of viral genes Tax, Rex and Gag By Western blot analysis (HTLV-1 protein). 5) Verify that the virus has spread to B cells through electron microscopy. 6) By imaging the expression of RFP and measuring the luciferase activity of the T cell reporter gene line, the displayed cells can spread from one HOS cell line to another host cell line and T cell reporter gene line.
Storage Conditions Liquid Nitrogen
Categories Drug Discovery Cells
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Cell Morphology Bipolar
Expression Profile δN-I-κBα , eGFP
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